THE EFFECTIVENESS OF RED BETEL LEAF (Piper crocatum) CLEANSING INFUSA IN REDUCING THE NUMBER OF TOTAL BACTERIA OF ULCER DIABETICUM ISOLATES IN ALLOXAN INDUCED WHITE RATS

Abstract

Background: Diabetes mellitus (DM) is a chronic disease that can cause complication diabetic ulcers, which have a long healing process. The wound management is very important here, especially the cleansing of the wound. The choice of techniques and solutions is very important, swabbing and irrigation are frequently used techniques. Normal saline is a standard solution used for the cleansing process, but an alternative solution is still needed for cleansing. One of it is red betel leaf (Piper crocatum) infusa. Red betel leaf contains flavonoids, phenols, tannins, and essential oils that can used as antiseptic. Objective: To determine the effectiveness of the using of red betel leaf infusion (Piper crocatum) in reducing the total bacterial number of diabetic ulcer isolates in white rats induced by alloxan. Method: the design in this study was true pre post control experiment. The subjects in this study were winstar type white rats that induced by alloxan and carried out injuries. The number of samples of 20 rats were divided into 5 groups. Samples were treated using irrigation and swabbing techniques using 20% red betel leaves and normal saline infusion for up to 5 interventions, each bacterial number was calculated and then processed by one way ANOVA test analysis. Results: the lowest bacterial number at the end of the intervention was 11 CFU / cm2 and the highest was 143CFU / cm2, the data were then analyzed using the ANOVA one-way test showing that the significance of cleansing with irrigation and swabbing techniques using 20% red betel leaf infusion. Results of Pos Hoct test with LSD obtained a p value of 0.001 for the cleaning group with a swabbing technique using 20% red betel leaf infusion. Conclusion: in this study cleansing with irrigation technique using 20% red betel leaf infusion and swabbing using 20% red betel leaf infusion was effective in decreasing the total number of bacterial numbers of diabetic ulcer isolates in white rats induced by alloxan.